Gibson Assembly® Primer-Bridge End Joining (PBnJTM) Cloning Gibson Assembly® Cloning without homologous overlap regions
نویسنده
چکیده
Gibson Assembly® cloning has proven to be useful as a molecular biology technique for the seamless assembly of synthetic and natural genes and large-scale genetic pathways. Notably, Gibson Assembly cloning has enabled the synthesis of the first bacterial genome1, the first synthetic cell2, and the first minimal cell3. Additionally, the Gibson Assembly method has been utilized for genetic recoding (genomewide codon removal)4, the engineering of Cre recombinase for improved site-specificity5, and has been incorporated into a one-step method for cloning gRNA for the CRISPR-Cas9 system6.
منابع مشابه
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تاریخ انتشار 2017